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Improved Alcohol Detection Testing - EtG
ETHYL GLUCURONIDE
Ethyl glucuronide (EtG) is a minor metabolite of ethanol (ethyl alcohol).
It is formed in vivo as a consequence of alcohol consumption. A small fraction
(0.02%) of a dose of alcohol is conjugated in the liver with glucuronic acid
to form ethyl glucuronide. This compound is excreted in the urine.
EtG can be detected in the blood for up to 36 hours and in the urine for
up to 5 days after heavy alcohol use. A positive finding of EtG in urine of
an
individual provides strong evidence that the person recently consumed alcohol,
even if the alcohol itself is no longer detectable. Therefore, this compound
is regarded as a biomarker of alcohol consumption and, potentially, relapses
of alcohol use by an individual who is under restriction.
Other biomarkers of alcohol use can be problematic since they can be influenced
by age, gender, a variety of other substances and non-alcohol-associated
disease. Fermentation may lead to false positives for alcohol since glucose
and yeast may be present in the urine. This is especially possible if a urine sample
is stored or shipped without refrigeration in warm weather. Since EtG is
only created during the metabolism of alcohol, there is no potential for
a wrong result due to external contamination.
Below is a list of the test specifications.
| Scope: |
Ethyl Glucuronide |
| Method of Analysis: |
Liquid Chromatography/Tandem Mass Spectrometry(LC/MS/MS) |
Specimen Requirement:
|
2mL Random UrineStability: Urine samples were reported to
be stable in storage for4 days at 4 °C or at room temperature (22 °C).
In addition, ethylglucuronide did not form in blank urine samples when
stored for14 days at 4 °C or at room temperature. |
Reporting Limits:
|
100/250/500 ng/mL to 25,000 ng/mLNote: All samples are qualitatively
screened and quantitativelyconfirmed |
| Reference: |
Wurst, F.M., S. Seidl, D. Ladewig, F. Muller-Spahn and A.
Alt (2002):"Ethyl glucuronide: on the time course of excretion in
urine duringdetoxification, " Addiction Biology 7: 427-434.Dahl, H.,
N. Stephanson, O. Beck and A. Helander (2002):"Comparison of urinary
excretion characteristics of ethanol and ethylglucuronide," J. Anal.
Toxicol. 26: 201-204.Stephanson, N., H. Dahl, A. Helander and 0. Beck (2002): "Directquantification
of ethyl glucuronide in clinical urine samples by liquidchromatography-mass
spectrometry," Ther. Drug Monitor. 24: 645-651. |
For further information please contact us at 800-732-3784, or via e-mail at mkt@firstlab.com.
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